| CAS No.: | 9012-36-6 |
|---|---|
| Formula: | C12h18o9 |
| EINECS: | 232-731-8 |
| Classification: | Biochemical Reagents |
| Grade: | Electrop Grade |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
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| Grouping | Separating materials and consumables |
| Keywords | Agarose |
| Brand | NJDULY |
| Model | I0004 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 100 per piece/Month |
| Packing | 100g/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
1. Basic information of REGULAR AGAROSE:
CAS#: 9012-36-6
Molecular formula: C12H18O9
Molecular weight: 306.26
Appearance: white powder
Characteristics: Glue strength (1.0%) (g/cm2): >1200
Storage conditions: dry and store at room temperature
Alias: Sepharose
Solubility: insoluble in cold water, soluble in boiling water, slowly soluble in hot water;
2. In the biological experiment, after adding agarose to the sample, the analysis of the reason why it does not solidify at room temperature:
1. The pH value of the sample is too low, partial acidity, hydrolysis, and agarose cannot coagulate when the pH value is less than 4.
2. The quality of the added agarose is not enough, so increase the dosage. Stirring is not uniform, and the solidification is not uniform if it is not fully shaken before pouring.
3. The sterilization temperature is too high and the heating time is too long. The pH changes or destroys the degree of solidification of agarose.
4. The sample will not solidify without heating and dissolving sufficiently.
Third, the difference and classification of agar powder, agarose, and low melting point agarose in biological experiments:
Agar powder is composed of agarose (agarose) and agar pectin (agaropectin). Agarose is a linear polymer. The basic structure is 1,3-linked β-D-galactofuranose and 1,4-linked 3,6-anhydro α-L-galactofuranose. Agar pectin is a heterogeneous mixture composed of many smaller molecules. Their structure is similar, but with sulfate and carboxyl components, the gelling ability is poor. Simply put, remove the agar pectin in the agar, and the remaining part is the agar in the industry. The color of agar varies from white to slightly yellow. It has a gum texture, no odor or a slight characteristic odor, and the agar is insoluble. In cold water, it is soluble in boiling water and slowly soluble in hot water.
Agarose is generally heated to above 90°C to dissolve in water, and a good semi-solid gel is formed when the temperature drops to 35-40°C. This is the main feature and basis for its multiple uses. The performance of agarose gel is usually expressed by gel strength. The higher the strength, the better the gel performance. The strength of better quality agarose is usually above 1200 g/cm2 (1% gel concentration). The gelation of agarose is caused by the existence of hydrogen bonds. Any factor that can destroy the hydrogen bonds can cause the destruction of the gelation. Agarose is hydrophilic and has almost no charged groups. It rarely causes denaturation and adsorption to sensitive biological macromolecules. It is an ideal inert carrier. In the process of agarose preparation, it is necessary to remove the agar pectin as much as possible, otherwise there may be traces of sulfate and pyruvate in the agarose instead of ionizing groups, which will cause electroendosmosis (EEO), which will cause the movement of particles influences. Agarose with better quality has a lower sulfate content, usually less than 0.2%, and electro-endosmosis is relatively small, usually less than 0.13.
Gel strength expression method:
Specifications Gel strength (1.5% concentration solution, 20 degrees Celsius, individual grams per square centimeter)
Low strength 150-400
Medium strength 401-800
High strength 801-1200
Super high strength greater than 1200
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
Q2:How to contact with us?
Answer:Click the Alibaba "Contact Supplier" And then send us message the product you interest in, you will get reply within 24 hours.
Q3:Can you give me a discount price?
Answer:Surely,It depend on your qty.
Q4: How can i get a sample?
Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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