| CAS No.: | No |
|---|---|
| Formula: | No |
| EINECS: | No |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
Suppliers with verified business licenses
Audited Supplier 
| Grouping | Enzyme |
| Keywords | T7 DNA Polymerase |
| Brand | NJDULY |
| Model | E0092 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 100 per piece/Month |
| Packing | 300U/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
Molecular weight: composed of 2 subunits, 804kDa polypeptide (T7 phage gene 5 expression product) and 12kDa thioredoxin (E. coli trxA gene expression product)
Grade: BR
Source: Two E. coli strains, one strain contains the T7 phage clone gene 5 and the other strain contains the E. coli clone gene trxA
Concentration: 10u/ul
Vitality definition: the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into the polynucleotide fragment (adsorbed on DE-81) within 30 minutes at 37°C
Enzyme activity analysis mixture: 40mM Tris-HCL (PH7.5), 1mM DTT, 10mM MgCL2, 0.1mg/ml BSA, 0.033mM various dNTPs, 0.4MBq/ML(3H)-dTTP and 0.5mM alkaline denatured cattle Thymus DNA
Storage buffer components: 20mM potassium phosphate (PH7.4), 0.1mM EDTA, 1mM DTT and 50% (v/v) glycerol
10× reaction buffer: 400mM Tris-HCL (PH7.5, 25ºC), 100mM MgCL2, 10mM DTT
Inhibitor: metal chelating agent, modification reagent (anhydrous acetic acid and N-ethyl maleimide can inactivate 3'→5' exonuclease, but does not affect the activity of polymerase
Inactivation: heating at 75ºC for 10 minutes
Quality control: Tests show no endonuclease contamination
Note for use: 37ºC analysis requires a short time incubation
Properties: suspension, recombinant enzyme. T7 DNA polymerase is a template-dependent DNA polymerase that catalyzes DNA synthesis in the 5'→3' direction. The DNA polymerase has a high continuous synthesis capacity and can continuously synthesize long fragments of DNA. The enzyme has 3'→5' exonuclease activity on single-stranded and double-stranded DNA
Uses: biochemical research. Removal of residual genomic DNA, purification of covalently closed-loop DNA molecules; long-length primer extension reaction; DNA 3'-end labeling; strand extension reaction of site-directed mutation sites; DNA 5'-overhang site filling; second strand cDNA Synthesis; in situ detection of apoptosis-related DNA fragments
Storage: -20°C
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
Q2:How to contact with us?
Answer:Click the Alibaba "Contact Supplier" And then send us message the product you interest in, you will get reply within 24 hours.
Q3:Can you give me a discount price?
Answer:Surely,It depend on your qty.
Q4: How can i get a sample?
Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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