| CAS No.: | 9014-74-8 |
|---|---|
| Formula: | No |
| EINECS: | 232-761-1 |
| Classification: | Biochemical Reagents |
| Grade: | Recombinant |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
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Audited Supplier 
| Grouping | Enzyme |
| Keywords |
Enteropeptidase |
| Brand | NJDULY |
| Model | E0174 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 50KU per Month |
| Packing | 100 iu/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
CAS No.: 9014-74-8
is a highly specific protease that recognizes the sequence of ASP ASP ASP Lys and hydrolyzes polypeptide at the C-terminal of Lys. It can transform trypsinogen into pancreatin, or it can cut the fusion protein with this recognition sequence. Because of its high specificity and high hydrolysis efficiency, enterokinase has been widely used in the development of genetic engineering products. One of its applications is as a tool protease for the specific cleavage of recombinant fusion protein, especially for the bioengineering pharmaceutical industry and the research of gene engineering, biochemistry, molecular biology, etc.
This product is a highly purified and active bovine enterokinase secreted and expressed by recombinant yeast, which has a wide range of applications (4-45oc, ph4.5-9.5), and still has some activities in the presence of various detergents and denaturants.
Enterokinase: enterokinase; enteropeptidase
Other names of enterokinase: peptidase; enteroproteinase; enteropeptidase; intestinal activator; ekase right is intestinal digestion
Level: Recombinant
Definition of enterokinase activity: 95% of thioredoxin-np-27, 0.5mg, is degraded to np-27 within 16h at 37oC, and the required enzyme quantity is defined as 1 active unit
Properties: sterilization liquid, recombinants
Main function: for biochemical research. Enterokinase is a serine protease, which can efficiently and specifically hydrolyze the fusion protein expressed by engineering bacteria (the recognition order is - (ASP) 4lys ↓ -) and release the target protein. Because enterokinase has the characteristics of high specificity and high hydrolysis efficiency, it is widely used in the development of genetic engineering products. It can be used as a protease to specifically identify and cut the substrate containing enterokinase cutting site. One of its applications is as a tool protease for the specific breaking of recombinant proteins, especially for Bioengineering pharmaceutical industry and genetic engineering Biochemistry, molecular biology, etc. However, the source of natural enterokinase is limited, and enterokinase extracted from animal tissues contaminates , which makes practical application difficult. This requires the production of high-purity enterokinase by genetic engineering. The high-purity enterokinase produced by genetic engineering is similar to the natural enzyme, but the cutting rate of enterokinase is faster.
Storage environment: - 20 ºC
There are many kinds of chemical reagents for enterokinase. There are different standards for classification and classification of chemical reagents in the world.
IUPAC classifies chemical reference materials as follows:
Class A: atomic weight standard.
Class B: the reference material closest to class A.
Grade C: standard reagent with content of 100 + - 0.02%
Grade D: standard reagent with content of 100 + - 0.05%
Grade E: reagent with purity determined by comparing grade C or D as standard
Chemical reagents can be divided into standard reagents, general reagents, biochemical reagents, etc. according to their uses. In our country, it is customary to call the C-grade and D-grade reagents equivalent to IUPAC standard reagents.
Common factors affecting the activity of recombinant enterokinase: biological reagent distribution company tulai biological
>2M urea,>250mM NaCl,>20mM b-ME,>0.1% SDS,>50mM imidazole.
If the sample solution contains one or more of the above components, in order to obtain the ideal results of enzyme cutting, please first dialysis the sample into 1 × reaction buffer, and then carry out enzyme cutting experiment.
·Enzyme digestion of recombinant bovine enterokinase:
Each reaction contained 15 μ g thioredoxin-np-27 and different amounts of enterokinase. After 16 hours of reaction at 37 ° C, SDS-PAGE gel at 15 ° C was used to detect the enzyme cutting effect. The enzyme content of each reaction is as follows:
Lane A: No rEK Lane B: 0.0008 units
Lane C: 0.0015 units Lane D: 0.003 units
Lane E: 0.008 units Lane F: 0.015 units Lane G: 0.03 units
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
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Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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