| CAS No.: | 8049-47-6 |
|---|---|
| Formula: | No |
| EINECS: | 232-468-9 |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
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Audited Supplier 
| Product Parameters | |
| Grouping | Enzyme |
| Keywords |
Pancreatin |
| Brand | NJDULY |
| Model | E0021 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity |
100 Piece/Pieces per Month |
| Other packaging |
Please contact customer service |
English Name: pancreatin; pancreatin from hog pancreas
Other names: pancreatin; pancreatin; pancreatin; pancreatin; pancreatin; pancreatin; pancreatin
CAS No.: 8049-47-6
Level: br
Molecular weight: about 23400
Titer: 1:4000
Trypsin activity: ≥ 4000U / g
Amylase activity of pancreas: ≥ 30000 U / g
Pancreatic lipase activity: ≥ 7000u / g
Loss on drying: ≤ 5.0%
Fat: ≤ 20mg / g
Dissolution method 1: the trypsin was dissolved with 90ml PBS without Ca or Mg ions, and then the PBS was added to 100ml (the enzyme was difficult to dissolve, and it could be dissolved overnight at 4 degrees if necessary). Adjust ph7.2 with time and 5.6% NaCO3 with time
Dissolution method 2: maintain the temperature of 80 ºC in the reaction pot for at least half an hour, and then pass the interlayer into tap water to reduce the temperature of the solution in the pot to 50 ºC. Add the required amount of pancreatin according to the enzyme / substrate ratio of 1:30, and start the enzymolysis reaction under the continuous agitation of the agitator. During this period, adjust the amount of steam flowing into the interlayer of the reaction pot to maintain the temperature at 50 ºC. In the process of enzymatic hydrolysis, in order to ensure the maximum biological activity of pancreatin and maintain the pH of the reaction solution at 8.0, it is necessary to continuously add NaOH dilute solution into the reaction solution. The enzymolysis was finished 6 hours after the addition of trypsin
Dissolution method 3: accurately weigh 8.00g of NaCl, 0.40g of KCl, 0.09g of na2hpo4.7h2o, 0.06g of KH2PO4, 0.01g of red, 0.35g of NaHCO3, dissolve them in about 800ml of five steam water, fix the volume to 1000ml after full dissolution, repack them, sterilize them and prepare d Hanks solution. Accurately weigh 0.125g pancreatin, dissolve it in 100ml d-hanks solution, stay overnight at 4 ºC, filter and repack it, and store it at - 20 ºC for standby
Properties: white or light yellow amorphous powder. It is a mixture of protease, lipase and amylase made from various animal pancreas. Hygroscopic. Insoluble in ethanol and ether. When the aqueous solution boils or meets with acid, it loses the enzyme activity. Its maximum activity is in neutral or slightly alkaline medium. Deactivation by mineral acid or excessive hydroxide or carbonate
Purpose: biochemical research. In neutral or weak alkaline environment (pH 6.8-8.5), trypsin can convert protein into peptone, amylase can convert starch into sugar, and lipase can decompose fat into glycerin and fatty acid
Storage: 2-8 ºC
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
Q2:How to contact with us?
Answer:Click the Alibaba "Contact Supplier" And then send us message the product you interest in, you will get reply within 24 hours.
Q3:Can you give me a discount price?
Answer:Surely,It depend on your qty.
Q4: How can i get a sample?
Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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