| CAS No.: | 9068-38-6 |
|---|---|
| Formula: | No |
| EINECS: | 232-964-5 |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
Suppliers with verified business licenses
Audited Supplier 
| Grouping | Enzyme |
| Keywords |
AMV Reverse transcriptase |
| Brand | NJDULY |
| Model | E0062 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 100 per piece/Month |
| Packing | 200U/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
CAS:9068-38-6
Grade: BR.
Source: Escherichia coli.
Definition of activity: an active unit is the amount of enzyme required to synthesize 1nmol dTNP into poly-nucleotide fragments (adsorbed on DE-81) at 37 ºC for 10 minutes.
Product description: AMV Reverse transcriptase (RT) is a recombinant avian myeloblastoma reverse transcriptase expressed by E. coli.
AMV RT is a heterodimer consisting of two different subunits (alpha and beta subunits).
AMV RT has a variety of activities, including RNA and DNA-dependent DNA polymerase activity, DNA-RNA unwinding activity, sequence-specific Mn2+-dependent endonuclease activity and RNase H (retention activity at 45-60 ºC. Therefore, reversible transcription contains a large number of secondary structure RNA.
Features: optimized active temperature range: 45-50 ºC; high thermal stability, reverse transcription temperature up to 60 ºC; efficient synthesis of 13kb-long first strand cDNA; and incorporation of modified nucleotides.
Functional testing: first chain cDNA synthesis and RT-PCR.
Preservation buffer components: 200mM potassium phosphate, 2mM DTT, 0.2% (Vamp V) Triton XMUE 100 and 50% (Vamp V) glycerol (PH7.2).
5 × AMV RT buffer: 250mM Tris-HCL (PH8.5,25 ºC), 40mM MgCL2, 150mM KCL, 5mM DTT.
Deactivation: when heated at 85 ºC for 5 minutes, it will be deactivated.
Character (the following information is for reference only): liquid, molecular weight about 157000 Dalton.
Inhibitors include metal chelating agents and inorganic phosphates.
Sodium pyrophosphate can reduce the activity of AMV RT, but when its concentration ≤ 4mM, it can increase the yield of full-length cDNA.
Use: this product is for scientific research only, not for other uses.
(for reference only) Synthesis of first strand cDNA for RT-PCR and real-time RT-PCR; can increase reaction temperature and reduce the effect of secondary structure on reverse transcription; synthesis of cDNA, for cloning and expression research; synthesis of labeled cDNA probe; analysis of RNA by primer extension.
Storage:-20 ºC
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
Q2:How to contact with us?
Answer:Click the Alibaba "Contact Supplier" And then send us message the product you interest in, you will get reply within 24 hours.
Q3:Can you give me a discount price?
Answer:Surely,It depend on your qty.
Q4: How can i get a sample?
Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
Suppliers with verified business licenses
Audited Supplier 
){kind=link}
){kind=link}