| CAS No.: | 9050-76-4 |
|---|---|
| Formula: | No |
| EINECS: | 1806241-263-5 |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
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Audited Supplier 
| Grouping | Enzyme |
| Keywords | Ribonulease H |
| Brand | NJDULY |
| Model | E0045 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 100 per piece/Month |
| Packing | 100U/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
CAS No.: 9050-76-4
Level: br
Molecular weight: 18.4kda, monomer
Source: E. coli mre-600 cells
Concentration: 2 ~ 5u / UL
Activity definition: one active unit refers to the amount of enzyme required to catalyze the production of 1 nmol acid soluble product at 37 ºC for 20 minutes
Enzyme activity analysis mixture: 20mm Tris HCl (pH7.8), 40mm KC1, 8mm MgCl2, 1mm DTT, 24um [3H] - poly (a) poly (DT), 0.03mg/ml BSA, 4% (V / V) glycerol
The storage buffer consisted of 25mm hepes-koh (ph8.0), 50mm KC1, 0.1mm EDTA, 1mm DTT, 0.1mg/ml BSA and 50% (V / V) glycerol
10 × reaction buffer: 20mm Tris HCl (pH7.8), 400mm KC1, 80mm MgCl2, 10mm DTT
Quality control: related tests showed that there was no internal or external DNA ribonuclease and ribonuclease contamination
Character: recombinant enzyme is a kind of ribonucleic acid endonuclease, it can specifically hydrolyze the RNA phosphodiester bond hybridized to DNA chain, so it can decompose RNA strand in RNA / DNA hybridization system. The enzyme cannot digest single or double stranded DNA. The inhibitors include metal chelates and sh blocking agents
Purpose: biochemical research. Methods: first strand cDNA was synthesized by removing mRNA, RT-PCR and QRT PCR; second strand cDNA was synthesized by removing RNA template; second strand cDNA was hybridized with oligo (DT) to remove poly (a) sequence in mRNA; site specific cleavage of RNA; in vitro study of polyadenylation reactants
Storage: - 20 ºC, heating at 65 ºC for 10 minutes will inactivate
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
Q2:How to contact with us?
Answer:Click the Alibaba "Contact Supplier" And then send us message the product you interest in, you will get reply within 24 hours.
Q3:Can you give me a discount price?
Answer:Surely,It depend on your qty.
Q4: How can i get a sample?
Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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