| CAS No.: | 9027-67-2 |
|---|---|
| Formula: | C4h10s3 |
| EINECS: | 232-819-6 |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose |
| Samples: |
|---|
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Audited Supplier 
| Grouping | Enzyme |
| Keywords | Terminal Transferase |
| Brand | NJDULY |
| Model | E0095 |
| Place of Origin | Nanjing, China (Mainland) |
| Payment | T/T |
| Port | Shanghai |
| Supply Capacity | 100 per piece/Month |
| Packing | 500U/piece |
| Other packaging | please consult customer service |
| To Find Out More, Click Here | |
CAS : 9027-67-2
Molecular weight: 60000
Grade: BR
Source: Escherichia coli cells, containing a gene encoding the end deoxynucleoside transferase of bovine thymus
Concentration: 20u/ul
Activity definition: refers to the amount of enzyme required to incorporate 1 nmol of deoxyribonucleic acid into a polynucleotide fragment (adsorbed on DE-81) within 60 minutes at 37°C
Enzyme activity analysis mixture: 66 mM potassium cacodylate (PH7.2), 1 mM CoCL2, 0.01% (v/v) Triton X-100, 10 uM oligo (dT) 10, 1 mM dTTP and 0.4 MBq/ml[3h]- dTTP
Storage solution components: 100 mM potassium acetate (PH6.8), 2 mM β-mercaptoethanol, 0.01% (v/v) Triton X-100 and 50% (v/v) glycerol
5× reaction buffer: 1M potassium cacodylate, 125mM Tris, 0.05% (v/v) Triton X-100, 5mM CoCL2 (PH7.2, 25ºC)
Inhibitors: metal chelating agents, ammonium, chloride, iodide and phosphate ions
Inactivation: add EDTA and heat at 70ºC for 10 minutes
Quality Assurance: Tests show no endonuclear and exonuclease ribonuclease, ribonuclease, phosphatase contamination
Note: Due to the presence of CoCL2, the TdT reaction buffer is not compatible with downstream applications. Column centrifugation extraction and ethanol precipitation should be used to purify the reaction mixture and remove CoCL2.
Properties: suspension, recombinant enzyme. Terminal transferase (TdT) is a template-free DNA polymerase that catalyzes the binding of deoxynucleotides to the 3'hydroxyl end of DNA molecules. Single and double-stranded DNA molecules with protruding, concave or smooth ends can be used as substrates for TdT. The general operation is: first open a single site on the vector, incubate it with the terminal transferase and a dNTP (such as dATP) to add tails, and the other foreign DNA fragment to be inserted is used to complement the same Adding dNTP (dTTP) with the same method. Next, the above two DNA fragments with complementary single-stranded tails are annealed to each other to form a hybrid vector to transform the recipient bacteria. The cracks or cuts in the hybrid carrier can be repaired by the whole bacteria.
Uses: biochemical research. Catalyze the addition of homopolymer at the 3'end of DNA; the 3'end label of DNA
Storage: -20°C
Nanjing Duly Biotech Co.,Ltd
has been established for 11 years. It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, nucleic acids, pigments, amino acids, vitamins,carbohydrates, buffers, surfactants,culture media and Separation of materials and kits.There are more than 10,000 products in total.
Our company's aim is to provide customers with the best quality products and the most intimate service.Personnel has been trained; every batch of material is checked; each procedure guarantees the quality; every bag of our products is top grade;service is thoughtful all the time.The quality, colors and forms of our products have reached a high level in related chemical industry, which relies on characteristic and advanced processing technology and it has gained a high and reliable reputation from customers at both quality and services.
Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2009.
Q2:How to contact with us?
Answer:Click the Alibaba "Contact Supplier" And then send us message the product you interest in, you will get reply within 24 hours.
Q3:Can you give me a discount price?
Answer:Surely,It depend on your qty.
Q4: How can i get a sample?
Answer:Free sample is available,but freight charges will be at your account and the charges will be return to you dudect from your order in future.
Q5:What are the main categories of products sold by the company?
Answer: It is mainly engaded in biological and chemical reagents,including enzyme reagents, proteins, antibiotics, plant hormones, nucleic acids, pigments, amino acids, vitamins, carbohydrates, buffers, surfactants, culture media and Separation of materials and kits.
Q6:Is the company mainly engaged in small packaging or large packaging?
Answer: Mainly do small packaging reagents, large packaging also do, specific can ask customer service personnel.
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